Reduced Nicotinamide Adenine Dinucleotide and Reduced Nicotinamide Adenine Dinucleotide Phosphate Diaphorase Activity in Human Polymorphonuclear Leukocytes
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منابع مشابه
Inhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.
Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
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Incubation of NADH at neutral and slightly alkaline pH leads to the gradual absorption of 1 mol of H+. This uptake of acid requires oxygen and mainly yields anomerized NAD+ (NAD+), with only minimal formation od acid-modified NADH. The overall stoichiometry of the reaction is: NADH + H+ + 1/2O2 leads to H2O + NAD+, with NADH peroxide (HO2-NADH+) serving as the intermediate that anomerizes and b...
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As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. Thes...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1974
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.10.3.528-534.1974